The extracellular juncture domains of Type 5 autotransporters

24 August 2022

Keynote, Live

• KN4 • 17:00 > 18:00 • The extracellular juncture domains of Type 5 autotransporters • Richelieu Amphithéâtre

17:00 • KN4 - 1 • The extracellular juncture domains of Type 5 autotransporters > J. Jens Preben MORTH
Content : Summary
Enterohemorrhagic and enteropathogenic Escherichia coli are among the most important food-borne pathogens. The virulence factor intimin is essential for attachment of pathogenic E. coli to intestinal host cells in human. Intimin is a surface exposed bacterial adhesion receptor which consists of four extracellular bacterial immunoglobulin-like (Big) domains, extending into the fifth subdomain which resemble a lectin like fold. The fifth domain binds to the Tir-receptor We have determined the crystal structures of the inner two Ig domains, called (D00 and D0) at 1.5 Å and the next domain pair D0-D1 at 1.8 Å resolution. With this we can confirm that the passenger of intimin has five distinct domains, only recently proposed. We describe that D00-D0 linker region exhibits a higher degree of rigidity and that D00 likely functions as a juncture domain exposed to the outer membrane and always anchored next to the inner membrane beta barrel inserted into the membrane. The accumulated data allows us to model the complete passenger of intimin and allows us to propose functionality through the degree of flexibility and rigidity inherent to the Big domains, D00-D0-D1, extending directly from the membrane

Julia Weikum,1,2 Alina Kulakova,3 Giulio Tesei,4 Shogo Yoshimoto,5 Line Vejby Jægerum,1 Monika Schütz,6 Katsutoshi Hori,5 Marie Skepö,7 Pernille Harris,3 Jack C. Leo,8,9 and J. Preben Morth1,2,10

1 Membrane Transport Group, Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo, P.O. Box 1137 Blindern, 0318 Oslo, Norway
2 Enzyme and Protein Chemistry, Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, 2800, Kgs. Lyngby, Denmark
3 Department of Chemistry, Technical University of Denmark, Kemitorvet building 207, 2800 Kgs. Lyngby, Denmark
4 Structural Biology and NMR Laboratory, Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, Copenhagen 2200, Denmark
5 Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
6 Interfaculty Institute for Microbiology and Infection Medicine, University Clinics Tübingen, 72076 Tübingen, Germany.
7 Division of Theoretical Chemistry, Department of Chemistry, Lund University, 221 00 Lund, Sweden
8 Department of Biosciences, University of Oslo, P.O. Box 1137 Blindern, 0318 Oslo, Norway.
9 Department of Biosciences, Nottingham Trent University, Nottingham NG11 8NS, UK
10 Institute for Experimental Medical Research (IEMR), Oslo University Hospital, Ullevål PB 4956 Nydalen, NO-0424 Oslo, Norway.